Role of multiplex polymerase chain reaction in diagnosing tubercular meningitis

Major criteria

(a) Clinically suspected patients with abnormal neurological signs and symptoms, for example, fever, neck stiffness, altered sensorium, and convulsions. (b) Positive CSF cytology and biochemical findings; pleocytosis (>10 leukocytes/mL), elevated protein concentration (>40 mg/dL), CSF glucose <60% of serum glucose and (c) Microbiologically positive (Ziehl–Neelsen smear positive for AFB or culture positive for M. tuberculosis and negative for other bacteria).

Minor criteria

(1) Close contact with a known case of active TB, (2) positive Mantoux skin test, (3) radiographic evidence of active TB at the extraneural site, (4) abnormal findings in computed tomography, and (5) clinical response to antitubercular drugs.

Based on above criteria, the patients were divided into three groups:

Group 1 (Definitive TBM group): Fulfilled major criteria A and B and any two of the minor criteria. Group 2 (Probable TBM group): Clinically suspected but do not fulfill two major and two minor criteria. Group 3 (Control group): Cases diagnosed with neurological pathology other than TBM.

All CSF samples were centrifuged at 3000 rpm for 5 min. Deposit was divided into three parts and subjected to smear microscopy by Ziehl–Neelsen staining, Middle Brook 7H9 broth culture, and multiplex PCR as per the standard guidelines. PCR was standardized with a standard strain of M. tuberculosis (H37Rv) which was found to have a sensitivity to detect the MTB DNA equivalent to 2–3 organisms. DNA extraction was done using commercial extraction kit as per manufacturer's (Qiagen, Germany) instructions. In each independent PCR assay, positive control and negative control were included. The positive control was the DNA of M. tuberculosis (H37Rv) and negative control included the PCR grade water. Identification of M. tuberculosis was done using primers designed to amplify 38 KD and IS6110 gene in the M. tuberculosis complex, and the expected target size was 340 bp and 245 bp, respectively. The sequence of primers used were KD1 (5'- CCA AGC AAG ATC CCG AGG GCT – 3'); KD2 (5’ – TTG ATG ATC GGG TAG CCG TCC 3') for 38 kDa gene (340 bp) and INS1 (5’ CGT GAG GGC ATC GAG GTG GC 3'); INS2 (5’ GCG TAG GCG TCG GTG ACA AA 3') for IS6110 gene (245 bp).[7]

In a 25 µl reaction, multiplex PCR was done for forty cycles including denaturation at 94°C for 1 min, annealing of primers at 65°C for 1 min and primer extension at 72°C for 1 min and one cycle of final extension at 72°C for 10 min. The amplification products were separated on 2% agarose gel and samples showing either the presence of both 340 and 245 bp bands or any one of the two bands under ultraviolet transilluminator (Vilber Lourmat, France) were considered positive [Figure 1]. The sensitivity, specificity, positive predictive, and negative predictive values (NPVs) of multiplex PCR were calculated.

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Figure 1

Gel photograph showing the result of multiplex polymerase chain reaction. Multiplex polymerase chain reaction with 38 kDa (340 bp) and IS6110 (245 bp) genes of Mycobacterium tuberculosis in cerebrospinal fluid samples. Lane M = Molecular marker, PC = axControl DNA of H37RV and 1–13 are sample

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As per the major and minor criteria, 26 (35.1%) patients were diagnosed with definitive TBM and 24 (32.4%) with probable TBM. Among patients with definitive TBM, 16 were positive by multiplex PCR (IS6110 and 38 kD) and M. tuberculosis was grown in two patients but AFB were not seen in any of the samples by microscopy. The samples (n = 2) showing growth of M. tuberculosis were also positive by PCR. Positivity of multiplex PCR was 61.5% and 29.2%, respectively, among definitive and probable group. Two of the 16 cases positive by PCR were negative for IS6110. Of the 26 definitive cases of TBM, radiological investigations suggestive of disease were observed in 15 (57.7%) patients.

Due to low culture positivity observed in the study, the results of multiplex PCR were compared with combination of clinical diagnosis and CSF adenosine deaminase (ADA) level of ≥10 U/L (considered as comparative standard).[8] Statistical analysis revealed sensitivity, specificity, positive predictive value, and NPV of 71.4%, 89.6%, 83.3%, and 81.2% [Table 1] and kappa coefficient of agreement as 0.6 for the multiplex PCR, respectively. Multiplex PCR detected two cases extra in TBM group.

Table 1 Comparison of multiplex polymerase chain reaction with clinical diagnosis and adenosine deaminase ≥10 U/L (comparative standard)

Although microscopy is very economical, it has the limitation of low sensitivity in extrapulmonary paucibacillary TB such as TBM. In the present study, all cases were AFB smear negative. The number of bacilli required for positive acid-fast staining is 10⁴/mL. Previous studies have reported sensitivity in the range of 0–10 for smear microscopy in TBM.[69] A recent study established that both CSF volume and duration of the microscopic evaluation are independently associated with bacteriological confirmation of CNS TB suggesting that a minimum of 6 mL of CSF fluid should be examined microscopically for a period of 30 min,[9] but invariably the laboratory receives only 1–2 mL of CSF.

In the present study, culture showed positivity of 8% only among the definitive TBM group. Other case series have also reported similar low CSF culture sensitivities varying from 4.3 to 48.9%.[610] Advanced molecular methods such as PCR is considered the most sensitive and specific diagnostic method, especially in cases where clinical suspicion is high but AFB staining is negative.[11] Panagariya et al. have reported sensitivity rates of PCR ranging from 65% to 85%.[12] The sensitivity of multiplex PCR in our study was 71.4%. The importance of multiplex PCR is shown earlier by Chauhan et al.[13] Twenty percent of strains showed low copy number, whereas 11% isolates lacked IS6110 element. Our PCR primers were directed against IS6110 gene and 38 kD protein for the detection of M. tuberculosis in the CSF. There were 2 (8%) samples in our study which showed the absence of IS6110 and presence of 38 kD amplicon in multiplex PCR indicating the presence of M. tuberculosis strains lacking IS6110. A similar study done by Kulkarni et al.[7] showed that there were 4 (6.25%) strains out of 64 samples which lacked IS6110 element. PCR was negative in ten cases among the definite TBM cases (n, 26) but all these patients responded to antitubercular therapy. The various reasons for PCR negativity in these cases could be attributed to small volume of CSF received in the laboratory, low number of bacteria in the CSF, poor lysis of bacteria in the CSF samples, or the presence of some PCR inhibitors.

In probable TBM group (n = 24), seven cases were PCR positive. Among them, four patients were planned for antitubercular therapy by the clinician based on PCR results and their clinical presentation, but two patients took discharge against medical advice because of financial constraint (one patient was HIV positive and another patient had higher ADA level of 19 U/L). These four cases can be considered as TBM because their clinical condition was not improving with other line of treatment. False positivity with PCR was observed in three cases as two were diagnosed with leptospirosis and one was diagnosed with scrub typhus. False positive results in PCR could be due to contamination during sample processing or amplicon contamination. To conclude, multiplex PCR for IS6110 and 38 kD is an effective tool for early diagnosis of TBM for prompt management of TBM cases.

Limitation of our study was not using MGIT or BACTEC culture for CSF samples which could have given better culture positivity. Another limitation could be inadequate amount of CSF samples.

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Conflicts of interest

There are no conflicts of interest.