Activity of cefotaxime versus ceftriaxone against pathogens isolated from various systemic infections: A prospective, multicenter, comparative, in vitro Indian study

Study setting

The present prospective, active controlled, in vitro study was conducted on different biological samples received in four microbiology laboratories for routine clinical testing from patients suffering from various infections wherein cefotaxime is routinely indicated. The study encompassed microbiology laboratories across different regions of India, including Bhubaneshwar in the east, Ahmedabad in the west, Delhi in the north, and Srikakulam in the south. Ethics committees of respective sites reviewed and approved the study protocol and corresponding documents. Samples of treatment naïve patients suffering from infections of the lower respiratory tract, urinary tract, skin, soft tissues; intra-abdominal infections (including peritonitis); genital infections caused by gonococci; acute meningitis, sepsis, and endocarditis were included if they were positive for bacterial culture. Patients who had a documented history of receiving antibiotics, antiviral agents, or interferon therapy within the last 5 days were excluded from the study. Samples that met the predefined inclusion and exclusion criteria were subjected to testing for antibiotic sensitivity of cefotaxime and ceftriaxone using strip and disc methods. This allowed for the determination of MIC and zone of inhibition (ZoI) for the respective antibiotics. Details of probable diagnosis were collected for each included sample. Ezy MIC strips and discs manufactured by HiMedia Laboratories, India were used to determine MIC and ZoI.

Culture and identification of bacteria

Culture media and inoculum were prepared as per standard methods. Each sample was inoculated on McConkey agar, chocolate agar, and blood agar. The inoculum on the plate was streaked out for discrete colonies with a sterile wire loop.

After inoculation, the culture plates were placed in an incubator at a temperature of 37°C for a duration of 24 hours. During this incubation period, the plates were regularly monitored for the growth of bacterial colonies. The isolated bacteria were subsequently subjected to morphological and biochemical tests, following the standardized procedure outlined by Cheesbrough.^[7] Positive samples were subjected to susceptibility testing by strips and discs methods.

Mic determination by strip test method

Ezy MIC strips is a quantitative technique that determines the MIC in μg/mL and antibiotic susceptibility of various aerobic and fastidious organisms using the concepts of dilution and diffusion principles for susceptibility testing. It directly quantifies antibiotic susceptibility in terms of discrete MIC values, which are more precise and reproducible as compared with conventional procedures. Ezy MIC strip is a thin, inert, and porous paper strip coated with antibiotic that has MIC reading scale in μg/mL over both sides of the strip. It also contains two or three-letter symbol printed on the top side, which represents the antibiotic. The antibiotic is dried and stabilized as a predefined exponential gradient with maximum concentration at one end and minimum at the other end, which covers a continuous concentration range across 15 twofold dilutions such as conventional method. There is gradual and effective transfer of the preformed antibiotic gradient from the strip into the agar medium when strip is applied to the medium. A stable, continuous, and exponential gradient of antibiotic concentrations is formed directly underneath the strip. The bacterial growth inhibition appeared as elliptical zones centered along the Ezy MIC strip, allowing for the determination of MIC values. The MICs were read from a scale printed on the strip, which covered a range of 0.016 to 256 μg/mL. Cefotaxime (test) and ceftriaxone (control) Ezy MIC strips manufactured by HiMedia that can measure MICs from 0.016 to 256 μg/mL were used in the study. The strips were placed on the plate using the applicator as per standard laboratory practice. Once positioned, the strips were not repositioned or adjusted, ensuring complete contact with the agar surface. The plates were then allowed to dry for 10 to 15 minutes and incubated them inverted under appropriate conditions. Readings were taken only when sufficient bacterial growth was observed. When growth occurred along the entire strip, MIC was read as > the highest values on the MIC strip, whereas in case of the inhibition ellipse is below the strip (did not intersect the strip), MIC was read as < the lowest value on the MIC scale.

Zone of inhibition using disk diffusion method

The disk diffusion method was used as described by Bauer et al.^[8] A 6-mm filter paper disk impregnated with a known concentration of an antimicrobial compound was placed on the agar plate. Complete contact with the agar surface of the disks was confirmed and agar plates were dried for 10 to 15 minutes. They were then incubated in an inverted position with appropriate conditions. When antibiotic concentration inhibits the growth and organism is sensitive, it forms a clear zone around the specific disc and thus creates a ZoI.

To assess the sensitivity of bacteria to antibiotics, the diameter of the ZoI was measured in millimeter (mm) using a caliper. The interpretation of the ZoI was done according to the guidelines provided by the Clinical and Laboratory Standards Institute (CLSI). For this purpose, antibiotic discs of cefotaxime and ceftriaxone, manufactured by HiMedia, were utilized to measure the ZoI.

Determining MIC₅₀

The MIC₅₀ represented the value at which ≥50% of the isolates in a test population were inhibited. Given “n” test strains and the values y1, y2…yn were representing a graded series of MICs starting with the lowest value, the MIC₅₀ was the value at position n × 0.5, when “n” was an even number of test strains and (n + 1) × 0.5 if “n” was an odd number of test strains.

INTERPRETATION OF RESULTS AS PER CLSI GUIDELINES

The MIC results and the ZoIs obtained for the cefotaxime and ceftriaxone were evaluated as per CLSI (M100 2022) guidelines and classified as sensitive/resistant (intermediate was considered as resistant for the study for available strains based on the MIC values and ZoI.^[9]

RESULTS

Total 400 samples were evaluated. The most common probable diagnosis was UTI (42.75%) followed by LRTI (20.75%), skin and soft tissue infection (16.75%), and sepsis (12.75%) (Figure 1). Urine (42.75%) was the most common sample followed by pus (17.5%), sputum (15.75%), blood (12.75%), endotracheal secretion (5%), and endocervical secretion (3.75%) (Figure 2). Among the 400 culture positive samples subjected to antibiotic sensitivity evaluation during the study, E. coli (47.75%) was the most common organism isolated followed by Klebsiella (26%), Salmonella (7.75%), and Proteus mirabilis (3.75%) (Figure 3).

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Figure 1:

Probable diagnosis of patients (values are in numbers, percentage). LRTI, lower respiratory tract infection; UTI, urinary tract infection.

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Figure 2:

Type of samples analyzed in the study (values are in numbers, percentage).

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Figure 3:

Distribution of isolated organisms from culture (values are in numbers, percentage).

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EFFICACY ANALYSIS

As shown in Table 1, mean MIC for E. coli, Klebsiella, Staphylococcus, Citrobacter koseri, and Serratia marcescens were lower for cefotaxime as compared with ceftriaxone, although not statistically significant. However, cefotaxime produced significantly (p < 0.05) more ZoI for E. coli, Klebsiella, and Salmonella as compared with ceftriaxone (Table 2).

MIC₅₀ values for each organism are shown in Table 3 for both cefotaxime and ceftriaxone.

Based on MIC and ZoI cutoff levels defined in CLSI guidelines, more proportions of E. coli, and Klebsiella were found to be sensitive to cefotaxime as compared with ceftriaxone, but it could not achieve the statistical significance. The sensitivity pattern of all organisms against cefotaxime and ceftriaxone as interpreted based on MIC and ZoI is shown in Figures 4 and 5, respectively.

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Figure 4:

Proportion of samples sensitive for cefotaxime versus ceftriaxone as per CLSI criteria based on MIC (values are in numbers, percentage). CLSI, clinical and Laboratory Standards Institute; MIC, minimum inhibitory concentration.

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Figure 5:

Proportion of samples sensitive for cefotaxime versus ceftriaxone as per CLSI criteria based on zone of inhibition (values are in numbers, percentage). CLSI, clinical and Laboratory Standards Institute.

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DISCUSSION

The current prospective, multicenter, in vitro study compared the sensitivity pattern between cefotaxime and ceftriaxone (third-generation cephalosporins) in India for various organisms isolated from clinical samples of patients indicated for treatment with cefotaxime/ceftriaxone. Up to the best of our knowledge, recent data on susceptible and resistant organisms from clinical isolates for cefotaxime and ceftriaxone are scarce from Indian region.

In this study, authors found that the mean MIC for E. coli, Klebsiella, Staphylococcus, C. koseri, and S. marcescens were lower for cefotaxime as compared with ceftriaxone, although not statistically significant. In addition, cefotaxime produced significantly (p < 0.05) more ZoI for E. coli, Klebsiella, and Aalmonella as compared with ceftriaxone. In the study conducted by Asari et al in 1983, cefotaxime showed higher antibacterial activity as compared with others for S. pneumoniae, S. pyogenes, S. agalactiae, E. coli, indole-positive Proteus, P. mirabilis, Klebsiella sp., S. marcescens, H. influenzae, and E. cloacae.^[10] Similar trends were found with cefotaxime when susceptibility of strains (S. aureus, S. pneumoniae, S. agalactiae, S. pyogenes, E. coli, E. cloacae, and P. morganii) from clinical isolates was compared with latamoxef, cefoperazone, and ceftizoxime.^[11,12] In recent study conducted in Bangladesh on uropathogens, 57% E. coli, 80% Klebsiella spp., 50% Enterobacter spp., 71% Proteus, and 57% Pseudomonas were found sensitive to cefotaxime.^[13]

Ceftriaxone and cefotaxime have similar antimicrobial spectrum and susceptibility of destruction to beta-lactamases, but have different serum protein binding capacity and elimination half-life. Ceftriaxone is 95% protein bound and its elimination half-life is 8.8 hours, whereas cefotaxime is less protein bound (35%) with elimination half-life of hours.^[5] Serum protein binding may inhibit the therapeutic efficacy by altering antibacterial activity and drug distribution. Thus, high serum protein binding of ceftriaxone may in fact limit its in vivo activity.^[14] The relationships between serum protein binding and the distribution of beta-lactam antibiotics have been extensively studied. In a study conducted by Nath et al, it is demonstrated that the bactericidal activity (in terms of survival rate of bacteria when exposed to a drug in broth containing 48 and 95% equivalent concentrations of serum albumin) of ceftriaxone was significantly lower as compared with cefotaxime (p < 0.001).^[5] In study conducted in Rwanda, the resistance pattern to cefotaxime and ceftriaxone was similar for hospital and community-acquired infections.^[15] In in vitro study conducted long back on major four species causing meningitis, MIC₉₀, and MBC₉₀ values were less for cefotaxime than ceftriaxone, and cefotaxime was found superior in terms of MBC₉₀.^[16] Clinically, cefotaxime and ceftriaxone show the comparable efficacy for various clinical conditions.^[17,18]

Various studies have also compared the changes in bacterial flora and adverse effects between cefotaxime and ceftriaxone. In a study by Bräutigam et al, differencebetweenpostoperative infection rates between cefotaxime and ceftriaxone was nonsignificant, but significant increase of Candida species in vaginal flora after 24 and 48 hours was noted in ceftriaxone group.^[19] They also found changes in thebowel flora, which was consistent with findings of other studies. Guggenbichler et al compared the impact of cefotaxime and ceftriaxone on the fecal microflora of pediatric patients. They observed that the first dose of ceftriaxone eradicated the susceptible enteric bacteria within 48 hours, leading to an overgrowth of Candida spp. and enterococci. Cefotaxime, however, had no essential suppressive effect.^[20] Additionally, many previous studies have reported high incidences of diarrhea with ceftriaxone, up to 12 to 59% during the therapy.^[21,22] In a study conducted by Scholz et al, it was observed that pseudolithiasis was commonly observed sonographically with ceftriaxone.^[23] This is a wellknown occurrence with this drug and is believed to relate mainly to the precipitation of the calcium salt of ceftriaxone after secretion into bile. However, there is no such incidence observed with cefotaxime. Similarly, in a study conducted by Schaad et al, it was observed that 46% patients develop biliary pseudolithiasis. In addition, among that one case developed urolithiasis with renal colic and obstructive ureteropyelectasia.^[24] This finding was later reconfirmed by many studies, where they observed formation of pseudolithiasis or sludge formation.^[25,26] In fact, this cholelithiasis can turn into cholecystitis and further complication. There are three different mechanisms by which a patient receiving ceftriaxone can develop acute cholecystitis: ceftriaxone-associated sludge can trigger existing gallstones to become symptomatic, ceftriaxone pseudolithiasis can transform into ceftriaxone gallstones, or the patient can become symptomatic from preexisting cholecystolithiasis unrelated to ceftriaxone therapy. Ceftriaxone is associated with thrombocytopenia with a definite or probable causal relationship.^[27] In pediatric patients, immune hemolytic anemia and biliary pseudolithiasis are major serious adverse effects found with ceftriaxone that can limit its use.^[28] Ceftriaxone is associated with more incidence of Clostridium difficile infections as it is excreted in bile and have more potential to disturb gut microbiota as compared with cefotaxime, which has renal elimination. The sudden decline in incidence of C. difficile infections was noted in Leipzig University Hospital, Germany with declined and increased uses of ceftriaxone and cefotaxime, respectively.^[29]

Incidence of HL-CASE Enterobacteriaceae was stabilized in France when use of ceftriaxone was replaced with cefotaxime, which was hypothesized to be associated with higher biliary elimination of ceftriaxone.^[30]

CONCLUSIONS

Cefotaxime can be preferred over ceftriaxone due to its pharmacokinetic, in vitro activity, and better safety profile for empirical treatment of various infections. The present in vitro comparative study findings provide further support and lead to the conclusion that cefotaxime exhibits a superior sensitivity profile compared with ceftriaxone. This superiority is observed in terms of various parameters such as MIC, ZoI, and the sensitivity pattern of isolated organisms. The study specifically focuses on patients with UTI, LRTI, skin and soft tissue infection, sepsis, genital infections, and intraabdominal infections.